anti-AATF antibody product blog
Tags: Antibody; AATF; anti-AATF antibody; Polyclonal Antibody; Che-1 (AATF) (Ser477);
The AATF aatf (Catalog #MBS502195) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Phospho Ser477 Che-1 (AATF) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Che-1 (AATF) (Ser477) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).Quality Control: Western blots performed on each lot.
WB: 1:1000. Researchers should empirically determine the suitability of the AATF aatf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The AATF aatf product has the following accession number(s) (GI #7657013) (NCBI Accession #NP_036270.1) (Uniprot Accession #Q9NY61). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Phospho Ser477 Che-1 (AATF) with the following immunoassay(s):
Testing Data
Affinity purified rabbit polyclonal antibody.
Antigen: Synthetic phospho-peptide surrounding the phospho Ser477 of human Che-1.
Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser477 conjugated to KLH
Immunogen Species: Human. Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, mouse, non-human primate, rat, sheep and Xenopus
Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with human tissue. It is also expected that the antibody will react with bovine, canine, mouse, non-human primates, rat, sheep and Xenopus as these species have 100% homology with the amino acid sequence used as antigen.
Biological Significance: Che-1, also known as AATF (apoptosis-antagonizing transcription factor), is a RNA polymerase II-binding protein involved in regulating the transcription factor E2F and promoting cell cycle progression (Burgdorf et al., 2004). It has been suggested that Che-1 may act as a neuroprotective factor against Abeta-induced apoptosis by suppressing the production of reactive oxidative species (Xie et al., 2004). The checkpoint kinase Chk2 has been shown to phosphorylate Che-1 at Ser477 contributing to the maintenance of the G2/M checkpoint induced by DNA damage (Bruno et al., 2006). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing AATF are readily searchable from our website. Different antibodies against the same target such as AATF may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.