anti-AATF antibody product blog
Tags: Antibody; AATF; anti-AATF antibody; Polyclonal Antibody; Protein AATF;
The AATF aatf (Catalog #MBS715767) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Protein AATF polyclonal Antibody, Biotin conjugated reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Protein AATF can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the AATF aatf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The AATF aatf product has the following accession number(s) (GI #7657013) (NCBI Accession #NP_036270.1) (Uniprot Accession #Q9NY61). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti-human Protein AATF polyclonal Antibody, Biotin conjugated with the following immunoassay(s):
Western Blot (WB) (Western blot All lanes: Protein AATF antibody at at 2 /mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 62kDa Observed band size: 50kDa Additional bands at: 100kDa?10 kDa. We are unsure as to the identity of these extra bands. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include.. � post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein � post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases � splice variants - alternative splicing may create different sized proteins from the same gene � relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.