anti-ACAP2 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-ACAP2 antibody; ACAP2; Centaurin beta 2;
The ACAP2 acap2 (Catalog #MBS642412) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Centaurin beta 2, CT (ACAP2, Ankyrin Repeat, ARFGAP with Coiled-coil ANK Repeat and PH Domains 2, CENTB2, Cnt b2, KIAA0041, Pleckstrin Homology Domains 2) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Centaurin beta 2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: ELISA: 1:32,000
Western Blot: 1:32,000 & 1-3ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml. Researchers should empirically determine the suitability of the ACAP2 acap2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ACAP2 acap2 product has the following accession number(s) (GI #119598433) (NCBI Accession #EAW78027.1) (Uniprot Accession #Q15057). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Centaurin beta 2, CT (ACAP2, Ankyrin Repeat, ARFGAP with Coiled-coil ANK Repeat and PH Domains 2, CENTB2, Cnt b2, KIAA0041, Pleckstrin Homology Domains 2) with the following immunoassay(s):
Immunohistochemistry (IHC) (Human Lung (formalin-fixed, paraffin-embedded) stained with CENTB2 antibody at 5ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)
Chemiluminescence (Antibody (1ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Centaurin beta 1 and Centaurin beta 2 are recruited to platelet-derived growth factor-induced dorsal membrane ruffles in NIH 3T3 mouse fibroblasts, and overexpression inhibited ruffle formation. In vitro, Centaurin beta 1 and Centaurin beta 2 preferred ARF6 as substrate rather than ARF1 or ARF5, and the GAP activity of both Centaurins was dependent upon phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). The isolated PH domain of mouse Centaurin beta 2 exhibits moderate affinity for PtdIns(3,5)P2, but it does not bind any other phosphoinositides tested, including PtdIns(4,5)P2. Mutation of a highly conserved arginine in both Centaurins result in lack of ARF-GAP activity. Overexpression in HeLa cells of either Centaurin blocks the formation of ARF6-dependent protrusions. Both Centaurins are also recruited to peripheral, tubular membranes, where activation of ARF6 occurs and allows membrane recycling back to the plasma membrane.
Immunogen: Synthetic peptide corresponding to C-EKLNRFQQDSQKF from human CENTB2 at C-terminal. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ACAP2 are readily searchable from our website. Different antibodies against the same target such as ACAP2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.