anti-ACTA2 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-ACTA2 antibody; ACTA2;
The ACTA2 acta2 (Catalog #MBS9125199) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ACTA2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s ACTA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).WB: 1:500 - 1:2000
IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the ACTA2 acta2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ACTA2 acta2 product has the following accession number(s) (GI #51316972) (NCBI Accession #P62736.1) (Uniprot Accession #P62736). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ACTA2 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using ACTA2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.)
Immunofluorescence (IF) (Immunofluorescence analysis of rat lung using ACTA2 antibody. Blue: DAPI for nuclear staining.)
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human ACTA2
Calculated Molecular Weight: 42kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ACTA2 are readily searchable from our website. Different antibodies against the same target such as ACTA2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Aortic Aneurysm, Thoracic, Breast Neoplasms, Cardiovascular Diseases, Disease Models, Animal, Fibrosis, Heart Diseases, Inflammation, Kidney Diseases, Liver Cirrhosis, Liver Diseases are some of the diseases may be linked to ACTA2 Polyclonal Antibody.