anti-ACTR3 antibody product blog
Tags: Antibody; ACTR3; anti-ACTR3 antibody; Polyclonal Antibody;
The ACTR3 actr3 (Catalog #MBS127721) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ACTR3 Polyclonal Antibody reacts with Human, Mouse, Monkey and may cross-react with other species as described in the data sheet. MyBioSource\'s ACTR3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).WB: 1:500 - 1:2000
IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the ACTR3 actr3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ACTR3 actr3 product has the following accession number(s) (GI #47117647) (NCBI Accession #P61158.3) (Uniprot Accession #P61158). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ACTR3 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using ACTR3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.)
Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells using ACTR3 antibody. Blue: DAPI for nuclear staining.)
Actin nucleation, the formation of new actin filaments from existing filaments, affects actin filament structure during cell motility, division, and intracellular trafficking. An important actin nucleation protein complex is the highly conserved ARP2/3 complex, consisting of ARP2, ARP3, and ARPC1-5. The ARP2/3 complex promotes branching of an existing actin filament and formation of a daughter filament following activation by nucleation-promoting factors, such as WASP/WAVE or cortactin (1). The formation of podosomes, small cellular projections that degrade the extracellular matrix, is enhanced by ARP2/3 complex action. ARP2/3 competes with caldesmon, an actin binding protein shown to negatively affect podosome formation (2). Along with N-WASP, the ARP2/3 complex regulates nuclear actin filament nucleation and controls actin polymerization during transcription (3).
Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human ACTR3
Calculated Molecular Weight: 47kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ACTR3 are readily searchable from our website. Different antibodies against the same target such as ACTR3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.