anti-ADCY3 antibody product blog
Tags: Antibody; Polyclonal Antibody; ADCY3; anti-ADCY3 antibody;
The ADCY3 adcy3 (Catalog #MBS415604) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s ADCY3 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB).The antibody can be diluted to 1:500-1,000 for immunofluorescence staining and 1:5,000-10,000 for western blotting. Researchers should empirically determine the suitability of the ADCY3 adcy3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ADCY3 adcy3 product has the following accession number(s) (GI #148536830) (NCBI Accession #NP_004027.2) (Uniprot Accession #O60266). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ADCY3 Antibody with the following immunoassay(s):
Western Blot (WB)
Immunogen: Adenylate cyclases are enzymes which interact with and are activated by the GTP bound alpha subunits of trimeric G-proteins. Activated adenylate cyclases are responsible for the production of the important "second messenger" signalling molecule cyclic-AMP, which is generated from ATP. The type III adenylate cyclase enzyme is localized in the membranes surrounding the cilia in neurons, and our antibody is an excellent marker of neuronal cilia in the brain and in cells in tissue culture. Adenylate cyclase type III is a large complex molecule of, in the human, 1145 amino acids with a deduced molecular weight of 129kDa. The protein may be variably glycosylated, so that on SDS-PAGE and western blots it runs as a diffuse band of about 160kDa in cortex and about 200kDa in olfactory epithelium (see below). The molecule has a complex structure, with 12 transmembrane domains and two cyclase domains. Each cyclase domain is immediately C-terminal to 6 transmembrane segments, but only the second, C-terminal cyclase is believed to be catalytically active. Our antibody was raised against a 20 amino acid peptide identical to the C-terminus of rat ACIII, which is PAAFPNGSSVTLPHQVVDNP. A cysteine residue was added to the N-terminus to allow coupling to MBS-activated keyhole limpet hemocyanin. The antibody works on human cells also, as the corresponding peptide in the human AC3 is the peptide LATFPNGPSVTLPHQVVDNS, which differs at only three amino acids. The HGNC name for this protein is ADCY3. Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ADCY3 are readily searchable from our website. Different antibodies against the same target such as ADCY3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.