anti-AGAP8 antibody product blog
Tags: Antibody; Polyclonal Antibody; AGAP8; anti-AGAP8 antibody;
The AGAP8 agap8 (Catalog #MBS9206649) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AGAP8 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s AGAP8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC).FC~~1:25
IF~~1:25
IHC~~1:25
WB~~1:1000. Researchers should empirically determine the suitability of the AGAP8 agap8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The AGAP8 agap8 product has the following accession number(s) (GI #74762218) (NCBI Accession #Q5SRD3.1) (Uniprot Accession #Q5SRD3). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the AGAP8 Antibody (C-term) with the following immunoassay(s):
Flow Cytometry (FC/FACS) (Flow cytometric analysis of MCF-7 cells using AGAP8 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9206649 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
Immunofluorescence (IF) (Fluorescent image of HeLa cells stained with AGAP8 Antibody (C-term). MBS9206649 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded H. liver section using AGAP8 Antibody (C-term). MBS9206649 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded M. liver section using AGAP8 Antibody (C-term). MBS9206649 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Western Blot (WB) (Western blot analysis of lysate from HT-1080 cell line, using AGAP8 Antibody (C-term). MBS9206649 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Putative GTPase-activating protein (Potential).
Antigen Type: Synthetic Peptide
Crown Antibody: Yes. Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing AGAP8 are readily searchable from our website. Different antibodies against the same target such as AGAP8 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.