anti-AGL antibody product blog
Tags: Antibody; Polyclonal Antibody; AGL; anti-AGL antibody;
The AGL agl (Catalog #MBS9201231) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AGL Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s AGL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA, Immunofluorescence (IF).WB~~1:1000
IF~~1:10~50. Researchers should empirically determine the suitability of the AGL agl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The AGL agl product has the following accession number(s) (GI #116734847) (NCBI Accession #NP_000019.2) (Uniprot Accession #P35573). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the AGL Antibody (C-term) with the following immunoassay(s):
Western Blot ( Anti-AGL Antibody (C-term) at 1:8000 dilution + human skeletal muscle lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 175 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB) (Western blot using anti-AGL (C-term) antibody (MBS9217121) at 1:1000 dilution. A total of 20 ug of lysates was loaded for each tissue. Data courtesy of Dr. Alan Cheng, Department of Internal Medicine, Life Sciences Institute, University of Michigan Medical Center, Ann Arbor, Michigan.)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of AGL Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF) (Expression of myc-GS causes wild type but not the ÄCBD mutant of AGL to aggregate around the PAS-stain-positive inclusions. HepG2 cells were transfected with either HA-tagged wild-type AGL (HA-AGL) or HA-AGL ÄCBD. Cells were fixed in formalin and processed for IF using anti-HA (green) and anti-myc (red) antibodies. White arrows indicate colocalization of HA-AGL and myc-GS.)
AGL is a glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and an amylo-1,6-glucosidase activity. Mutations in the AGL gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing.
Function: Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4- alpha-D-glucan4-alpha-D- glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.
Cellular Location: Cytoplasm. Note=Under glycogenolytic conditions localizes to the nucleus
Tissue Location: Liver, kidney and lymphoblastoid cells express predominantly isoform 1; whereas muscle and heart express not only isoform 1, but also muscle-specific isoform mRNAs (isoforms 2, 3 and 4). Isoforms 5 and 6 are present in both liver and muscle.