|The AK2 ak2 (Catalog #MBS129066) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AK2 Polyclonal Antibody reacts with Human, Mouse, Rat, Zebrafish and may cross-react with other species as described in the data sheet. MyBioSource\'s AK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP).
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50. Researchers should empirically determine the suitability of the AK2 ak2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The AK2 ak2 product has the following accession number(s) (GI #1708596) (NCBI Accession #P54819.2) (Uniprot Accession #P54819). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the AK2 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of HeLa cells, using AK2 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.)
Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2.
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human AK2
Calculated Molecular Weight: 26kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing AK2 are readily searchable from our website. Different antibodies against the same target such as AK2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Anemia, Deafness, Death, Hyperplasia, Immune System Diseases, Nervous System Diseases, Reticular dysgenesis, Severe Combined Immunodeficiency, Vascular Diseases, Weight Loss are some of the diseases may be linked to AK2 Polyclonal Antibody. The following patways have been known to be associated with this gene.