anti-AKR1B1 antibody product blog
Tags: Antibody; Polyclonal Antibody; AKR1B1; anti-AKR1B1 antibody;
The AKR1B1 akr1b1 (Catalog #MBS624440) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AKR1B1, CT (Aldose Reductase, AR, Aldehyde Reductase, Aldo-keto Reductase Family 1 Member B1, ALDR1) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s AKR1B1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50. Researchers should empirically determine the suitability of the AKR1B1 akr1b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The AKR1B1 akr1b1 product has the following accession number(s) (GI #4502049) (NCBI Accession #NP_001619.1) (Uniprot Accession #P15121). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
AKR1B1, CT (Aldose Reductase, AR, Aldehyde Reductase, Aldo-keto Reductase Family 1 Member B1, ALDR1)
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the AKR1B1, CT (Aldose Reductase, AR, Aldehyde Reductase, Aldo-keto Reductase Family 1 Member B1, ALDR1) with the following immunoassay(s):
Western Blot (WB) (AKR1B1 Antibody (C-term) western blot analysis in A375 cell line and human placenta tissue lysates (35ug/lane).This demonstrates the AKR1B1 antibody detected the AKR1B1 protein (arrow).)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AKR1B1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of AKR1B1 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
AKR1B1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This protein catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol.
Immunogen: Synthetic peptide selected from the C-terminal region of human AKR1B1 (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing AKR1B1 are readily searchable from our website. Different antibodies against the same target such as AKR1B1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.