anti-Akt antibody product blog
Tags: Antibody; anti-Akt antibody; Akt; Polyclonal Antibody;
The Akt akt1 (Catalog #MBS9410609) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Akt (Phospho-Ser129) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Akt can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).Western blotting: 1:500~1:1000. Researchers should empirically determine the suitability of the Akt akt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Akt akt1 product has the following accession number(s) (GI #62241013) (NCBI Accession #NP_001014431.1) (Uniprot Accession #P31749). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Akt (Phospho-Ser129) Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts from A549 cells treated with PMA using Phospho-Akt (Ser129) antibody.The lane on the right is treated with the antigen-specific peptide.)
General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at \'Ser-939\' and \'Thr-1462\', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. Nguyen le XT, Mitchell BS (2013) Proc Natl Acad Sci U S A 110, 20681-6 Ponce DP, et al. (2011) Mol Cell Biochem 356, 127-32 Siddiqui-Jain A, et al. (2010) Cancer Res 70, 10288-98.
Purity: Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Immunogen Type: Peptide-KLH
Immunogen Description: Peptide sequence around phosphorylation site of serine129(D-N-S(p)-G-A) derived from Human Akt. Modification: Phospho-Ser129
Target Name: Akt. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Akt are readily searchable from our website. Different antibodies against the same target such as Akt may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.