|The AKT1 akt1 (Catalog #MBS845914) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-AKT1 (Tyr474) polyconal Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s AKT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunoblot; ELISA (EIA).
Immunoblot: 0.5-2 mug/ml. Researchers should empirically determine the suitability of the AKT1 akt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The AKT1 akt1 product has the following accession number(s) (GI #62241013) (NCBI Accession #NP_001014431.1) (Uniprot Accession #P31749). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Polyclonal antibody to detect AKT1 (Phospho-Tyr474) in human samples.
The serine/threonine kinase Akt family contains several members; including Akt1 (also designated PKB or RacPK); Akt2 and Akt 3; which exhibit sequence homology with the protein kinase An and C families and are encoded by the c-Akt proto-oncogene. They have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-? tyrosine residues 740 and 751; which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity; and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus; Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase (s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin; suggesting that the protein signals downstream of the PI kinases.
Immunogen: Synthetic peptide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing AKT1 are readily searchable from our website. Different antibodies against the same target such as AKT1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. AKT1 also interacts with the following gene(s): AKT2, BAD, CASP3, CASP9, FOXO1, IRS1, MTOR, NOS3, PTEN, SRC. Breast Neoplasms, Cardiovascular Diseases, Drug Toxicity, Inflammation, Lung Neoplasms, Necrosis, Neoplasms, Neoplasms, Experimental, Nervous System Diseases, Prostatic Neoplasms are some of the diseases may be linked to Phospho-AKT1 (Tyr474) polyconal Antibody.