anti-ALCAM antibody product blog
Tags: Antibody; ALCAM; Polyclonal Antibody; anti-ALCAM antibody; Activated Leukocyte Cell Adhesion Molecule (ALCAM);
The ALCAM alcam (Catalog #MBS2015695) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Activated Leukocyte Cell Adhesion Molecule (ALCAM) reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Activated Leukocyte Cell Adhesion Molecule (ALCAM) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB).Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ALCAM alcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ALCAM alcam product has the following accession number(s) (GI #31791059) (NCBI Accession #NP_033785.1) (Uniprot Accession #Q61490). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- WYT VNSAYGDTIV MPCRLDVPQN LMFGKWKYEK PDGSPVFIAF RSSTKKSVQY DDVPEYKDRL SLSENYTLSI ANAKISDEKR FVCMLVTEDN VFEAPTLVKV FKQPSKPEIV NKAPFLETDQ LKKLGDCISR DSYPDGNITW YRNGKVLQPV EGEVAILFKK EIDPGTQLYT VTSSLEYKTT RSDIQMPFTC SVTYYGPSGQ KTIYSEQEIF DIYYPTEQVT IQVLPPKNAI KEGDNITLQC LGNGNPPPEE FMFYLPGQPE GIRSSNTYTL TDVRRNATGD YKCSLIDKRN MAASTTITVH YLDLSLNPSG EVTKQIGDTL PVSCTISASR NATVVWMKDN IRLRSSPSFS SLHYQDAGNY VCETALQEVE GLKKRESLTL IVEGKPQIKM TKKTDPSGLS KTIICHVEGF PKPAIHWTIT GSGSVINQTE ESPYINGRYY SKIIISPEEN VTLTCTAENQ LERTVNSLNV SAISIPEHDE ADDISDENRE.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Polyclonal Antibody to Activated Leukocyte Cell Adhesion Molecule (ALCAM) with the following immunoassay(s):
Western Blot (WB) (Western Blot: Sample. Lane1: Mouse Brain Tissue; Lane2: Mouse Liver Tissue.)
Western Blot (WB) (Western Blot: Sample: RecombinantALCAM,Mouse.)
Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Stomach Tissue))
Knockout Validation (Knockout Validation: Lane 1: Wild-type HT1080 cell lysate; Lane 2: ALCAM knockout HT1080 cell lysate; Predicted MW: 65kd Observed MW: 100kd Primary Ab: 3ug/ml Rabbit Anti-Mouse ALCAM Antibody Second Ab: 0.2ug/mL�HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Immunogen: Recombinant ALCAM (Trp28~Lys527) expressed in E.coli.
Cross Reactivity: Human, Mouse, Rat. Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2034734. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ALCAM are readily searchable from our website. Different antibodies against the same target such as ALCAM may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.