anti-ALOX12 antibody product blog
Tags: Antibody; Polyclonal Antibody; ALOX12; anti-ALOX12 antibody;
The ALOX12 alox12 (Catalog #MBS9204137) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ALOX12 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s ALOX12 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC).IF~~1:100
WB~~1:1000. Researchers should empirically determine the suitability of the ALOX12 alox12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ALOX12 alox12 product has the following accession number(s) (GI #154426292) (NCBI Accession #NP_000688.2) (Uniprot Accession #P18054). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ALOX12 Antibody (C-term) with the following immunoassay(s):
Immunofluorescence (IF) (Immunofluorescent analysis of A549 cells, using ALOX12 Antibody (C-term). MBS9204137 was diluted at 1?100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).)
Western Blot (WB) (Western blot analysis of ALOX12 Antibody (C-term) in K562 cell line lysates (35ug/lane). ALOX12 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human skin tissue reacted with ALOX12 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (ALOX12 Antibody (C-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Oxygenase and 14,15-leukotriene A4 synthase activity.
Antigen Type: Synthetic Peptide
Crown Antibody: Yes. Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ALOX12 are readily searchable from our website. Different antibodies against the same target such as ALOX12 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Connective Tissue, Embryonic Tissue, Intestine, Lung, Muscle, Ovary, Skin tissues are correlated with this protein.