anti-ALPL antibody product blog
Tags: Antibody; ALPL; Polyclonal Antibody; anti-ALPL antibody; Phosphatase, Alkaline, Tissue-nonspecific Isozyme;
The ALPL n/a (Catalog #MBS626926) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phosphatase, Alkaline, Tissue-nonspecific Isozyme (AP-TNAP, ALPL, TNSALP, Alkaline Phosphatase Liver/Bone/Kidney Isozyme) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Phosphatase, Alkaline, Tissue-nonspecific Isozyme can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot:1-2ug/ml
Immunohistochemistry (paraffin):1-2ug/ml. Researchers should empirically determine the suitability of the ALPL n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ALPL n/a product has the following accession number(s) (GI #545490188) (NCBI Accession #XP_005617271.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Phosphatase, Alkaline, Tissue-nonspecific Isozyme (AP-TNAP, ALPL, TNSALP, Alkaline Phosphatase Liver/Bone/Kidney Isozyme) with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Rat Tissue sections (samll intestine) Staining ALPL in cytoplasm DAB chromogenic reaction)
Western Blot (WB) (Western blot analysis of Hela cell Ttissue lysis Using ALPL antibody)
Alkaline phosphatase (ALPL) removes phosphate groups from the 5\' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts,the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
Immunogen: Synthetic peptide corresponding to a sequence at the N-terminal of human ALPL, different from the related rat and mouse sequence by two aa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ALPL are readily searchable from our website. Different antibodies against the same target such as ALPL may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.