anti-ALPL antibody product blog
Tags: Antibody; ALPL; Polyclonal Antibody; anti-ALPL antibody;
The ALPL alpl (Catalog #MBS9202710) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ALPL Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s ALPL can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), ImmunohistochemistrypParaffin (IHC-P-Leica), Western Blot (WB), Immunofluorescence (IF).IHC-P-Leica~~1:500
WB~~1:2000
IF~~1:10~50
FC~~1:10~50. Researchers should empirically determine the suitability of the ALPL alpl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ALPL alpl product has the following accession number(s) (GI #116734717) (NCBI Accession #NP_000469.3) (Uniprot Accession #P05186). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ALPL Antibody (Center) with the following immunoassay(s):
Immunohistochemical (IHC) (Immunohistochemical analysis ofparaffin-embedded human kidney tissue using MBS9202710 performed on the Leica� BOND RXm.
Samples were incubated with primary antibody(1/500) for 1 hours at room temperature.
An undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
Immunohistochemical (IHC) (Immunohistochemical analysis of paraffin-embedded human liver tissue using MBS9202710 performed on the Leica� BOND RXm.
Samples were incubated with primary antibody(1/500) for 1 hours at room temperature.
An undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
Western Blot (WB) (Anti-ALPL Antibody (Center) at 1:2000 dilution + F9 whole cell lysate Lysates/proteins at 20 ug per lane.
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB) (All lanes : Anti-ALPL Antibody (Center) at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: F9 whole cell lysate Lysates/proteins at 20 ug per lane.
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 57 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunofluorescence (Confocal immunofluorescent analysis of ALPL Antibody (Center)(Cat# MBS9202710) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Flow Cytometry (ALPL Antibody (Center) (Cat. MBS9202710) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The genes for the first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. This protein is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to a disorder known as hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms.