anti-AMPK1, 2 antibody product blog
Tags: Antibody; Polyclonal Antibody; AMPK1, 2; anti-AMPK1, 2 antibody;
The AMPK1, 2 n/a (Catalog #MBS625033) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AMPK1, 2 (5\'-AMP-activated Protein Kinase Catalytic Subunit alpha-1, AMPK Subunit alpha-1, PRKAA1/5\'-AMP-activated Protein Kinase Catalytic Subunit alpha-2, AMPK Subunit alpha-2, PRKAA2) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s AMPK1, 2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC).Suitable for use in Immunohistochemistry.
Dilution: Immunohistochemistry: 1:50-1:100. Researchers should empirically determine the suitability of the AMPK1, 2 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis. AMPK is a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits, each of which is encoded by two or three distinct genes (a1, 2; b1, 2; g1, 2, 3). The kinase is activated by an elevated AMP/ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia and ischemia. The tumor suppressor LKB1, in association with accessory proteins STRAD and MO25, phosphorylates AMPK-alpha at Thr172 in the activation loop and this phosphorylation is required for AMPK activation. AMPK-alpha is also phosphorylated at Thr258 and Ser485 (for a1; Ser491 for a2). The upstream kinase and the biological significance of these phosphorylation events have yet to be elucidated. The beta1 subunit is post-translationally modified by myristoylation and multi-site phosphorylation including Ser24/25, Ser96, Ser101, Ser108 and Ser182. Phosphorylation at Ser108 of the beta1 subunit seems to be required for the activation of AMPK enzyme, while phosphorylation at Ser24/25 and Ser182 affects AMPK localization. Several mutations in AMPK-gamma subunits have been identified, most of which are located in the putative AMP/ATP binding sites (CBS or Bateman domains). Mutations at these sites lead to reduction of AMPK activity and cause glycogen accumulation in heart or skeletal muscle. Accumulating evidence indicates that AMPK not only regulates the metabolism of fatty acids and glycogen, but also modulates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS.
Immunogen: Synthetic non-phosphopeptide corresponding to human AMPK1/AMPK2 around the phosphorylation site of serine 485/491 (S-G-SP-V-S). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing AMPK1, 2 are readily searchable from our website. Different antibodies against the same target such as AMPK1, 2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.