anti-AP2M1 antibody product blog
Tags: Antibody; Polyclonal Antibody; AP2M1; anti-AP2M1 antibody; AP-2 complex subunit mu;
The AP-2 ap2m1 (Catalog #MBS715296) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human AP-2 complex subunit mu polyclonal Antibody, HRP conjugated reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s AP-2 complex subunit mu can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the AP-2 ap2m1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The AP-2 ap2m1 product has the following accession number(s) (GI #68799814) (NCBI Accession #NP_001020376.1) (Uniprot Accession #Q96CW1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti-human AP-2 complex subunit mu polyclonal Antibody, HRP conjugated with the following immunoassay(s):
Western Blot (WB) (Western blot All lanes: AP-2 complex subunit mu antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 48 kDa Observed band size: 22kDa Additional bands at: 88 kDa.We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include.. � post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein � post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases � splice variants - alternative splicing may create different sized proteins from the same gene � relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Storage Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate: HRP. Immunogen: Recombinant human AP-2 complex subunit mu protein
Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-49150 / sc-49151 / sc-49152 / sc-99026. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing AP-2 are readily searchable from our website. Different antibodies against the same target such as AP-2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.