|The APH1A aph1a (Catalog #MBS150469) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The APH1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s APH1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB).
APH1 antibody can be used for detection of APH1 by Western blot at 0.5 - 2 mug/mL. Despite its predicted molecular weight, APH1 protein often migrates at aberrant locations in SDS-PAGE. Researchers should empirically determine the suitability of the APH1A aph1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The APH1A aph1a product has the following accession number(s) (GI #14250557) (NCBI Accession #AAH08732) (Uniprot Accession #Q96BI3). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the APH1 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of APH1 in RAW264.7 cell lysate with APH1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.)
APH1 Antibody: APH1 was initially identified as a component of the Notch pathway in C. elegans. Along with nicastrin, PEN2, and presenilin-1 APH1 is an essential component of the gamma-secretase complex which cleave the amyloid precursor protein (APP) at what are known as the gamma- and epsilon-sites and can lead to the accumulation of the Amyloid beta peptide (Abeta) cleavage product that is associated with Alzheimer\'s disease. APH1 exists in at least three distinct isoforms with APH1a as the principal isoform present in the gamma-secretase complex. Mice deficient in this isoform, but not the other two, were lethal at E10.5, with impaired vascular and neural development observed.
Immunogen: APH1 antibody was raised against a 13 amino acid synthetic peptide from near the amino terminus of human APH1a.
Buffer: APH1 Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing APH1A are readily searchable from our website. Different antibodies against the same target such as APH1A may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.