anti-Apitd1 antibody product blog
Tags: Antibody; Polyclonal Antibody; APITD1; anti-APITD1 antibody;
The Apitd1 apitd1 (Catalog #MBS6005280) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The APITD1, ID (APITD1, CENPS, FAAP16, MHF1, Centromere protein S, Apoptosis-inducing TAF9-like domain-containing protein 1, FANCM-interacting histone fold protein 1, Fanconi anemia-associated polypeptide of 16kD) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s APITD1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS).Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the Apitd1 apitd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Apitd1 apitd1 product has the following accession number(s) (GI #28913612) (NCBI Accession #AAH48521.1) (Uniprot Accession #Q9D084). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the APITD1, ID (APITD1, CENPS, FAAP16, MHF1, Centromere protein S, Apoptosis-inducing TAF9-like domain-containing protein 1, FANCM-interacting histone fold protein 1, Fanconi anemia-associated polypeptide of 16kD) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane) using MBS6005280. APITD1 (arrow) was detected using MBS6005280.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis in formalin fixed and paraffin embedded brain tissue using MBS6005280 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6005280 for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of Jurkat cells (right histogram) using MBS6005280 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
APITD1 was identified in the neuroblastoma tumour suppressor candidate region on chromosome 1p36. It contains a TFIID-31 domain, similar to that found in TATA box-binding protein-associated factor, TAF(II)31, which is required for p53-mediated transcription activation. This gene was expressed at very low levels in neuroblastoma tumours, and was shown to reduce cell growth in neuroblastoma cells, suggesting that it may have a role in a cell death pathway.
Immunogen: APITD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 50-80 from the Central region of human APITD1. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Apitd1 are readily searchable from our website. Different antibodies against the same target such as Apitd1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.