anti-ApoE3 antibody product blog
Tags: Antibody; Polyclonal Antibody; ApoE3; anti-ApoE3 antibody;
The ApoE3 apoe (Catalog #MBS695138) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-human ApoE3 reacts with HumanCross Reactivity: human ApoE2, human ApoE and may cross-react with other species as described in the data sheet. MyBioSource\'s ApoE3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB).
Sandwich ELISA: To detect hApoE3 by sandwich ELISA (using 100 mul/well antibody solution) a concentration of 0.5 - 2.0 mug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human ApoE3 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hApoE3.
Western Blot: To detect hApoE3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hApoE3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Researchers should empirically determine the suitability of the ApoE3 apoe for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ApoE3 apoe product has the following accession number(s) (GI #114039) (NCBI Accession #P02649.1) (Uniprot Accession #P02649). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hApoE3. Anti-Human ApoE3 specific antibody was purified by affinity chromatography employing immobilized hApoE3 matrix.
In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ApoE3 are readily searchable from our website. Different antibodies against the same target such as ApoE3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. ApoE3 also interacts with the following gene(s): APOA2, APOB, APOC3, LDLR, LPL, LRP1, LRP8, MAPT, SCARB1, VLDLR. The following patways have been known to be associated with this gene. Bone, Brain, Heart, Kidney, Liver, Lung, Muscle, Nerve, Spleen, Vascular tissues are correlated with this protein. Alzheimer Disease, Arteriosclerosis, Atherosclerosis, Cardiovascular Diseases, Cognition Disorders, Coronary Disease, Hypercholesterolemia, Hyperlipidemias, Inflammation, Plaque, Atherosclerotic are some of the diseases may be linked to Anti-human ApoE3.