|The Aqp6 aqp6 (Catalog #MBS624426) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The Anti-Aquaporin 6 (WCH3, hKID, AQP2-L) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Aquaporin 6 can be used in a range of immunoassay formats including, but not limited to, ELISA; Western Blot.
Suitable for use in Immunofluorescence, ELISA, Western Blotting, Immunohistochemistry.
Recommended Dilutions: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using Chemiluminescence technique.
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml.
Immunohistochemistry & Immunofluorescence: we recommend the use of affinity purified antibody at 2-10ug/ml in paraformaldehyde fixed sections of tissues. Neat serum can be used at 1:500 or more. Researchers should empirically determine the suitability of the Aqp6 aqp6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Aqp6 aqp6 product has the following accession number(s) (GI #110832717) (Uniprot Accession #Q14BV5). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporin. The lens specific AQP0 represents up to 80% of total lens membrane protein. Defects in MIP26 are cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an amino acid substitution that inhibits targeting of MIP to the cell membrane.
Immunogen: A 19 AA synthetic peptide within the carboxy terminal domain of human AQP6 (1). This domain is predicted to be cytoplasmic.
Source Information: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Aqp6 are readily searchable from our website. Different antibodies against the same target such as Aqp6 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.