anti-ARF4 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-ARF4 antibody; ARF4;
The ARF4 arf4 (Catalog #MBS248291) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Polyclonal to Human ARF4 reacts with Bat, Gibbon, Bovine, Chimpanzee, Chicken, Dog, Guinea pig, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Rabbit, Rat, Sheep and may cross-react with other species as described in the data sheet. MyBioSource\'s ARF4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA).ELISA (1:128000), IHC-P (5 ug/ml), WB (0.3 - 1 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 19kD band observed in lysates of cell line HeLa (calculated MW of 20.5kD according to NP_001651.1). Recommended concentration: 0.3-1 ug/ml. Researchers should empirically determine the suitability of the ARF4 arf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ARF4 arf4 product has the following accession number(s) (GI #4502205) (NCBI Accession #NP_001651.1) (Uniprot Accession #P18085). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat Polyclonal to Human ARF4 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-ARF4 antibody IHC staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Western Blot (WB) (ARF4 antibody (0.5 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Target Species: Human
Immunogen Description: Synthetic peptide C-SEMTDKLGLQSLRN from an internal region of human ARF4 (NP_001651.1). Immunogen Type: Synthetic peptide
Immunogen: ARF4 antibody was raised against synthetic peptide C-SEMTDKLGLQSLRN from an internal region of human ARF4 (NP_001651.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Bat, Dog, Bovine, Horse, Rabbit, Porcine, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Lizard (100%); Platypus, Xenopus (93%); Felinefish, Zebrafish (86%).
Antigen Modification: aa137-150. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ARF4 are readily searchable from our website. Different antibodies against the same target such as ARF4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. ARF4 also interacts with the following gene(s): ASAP1, EGFR, PKD1.