anti-ARHGEF18 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-ARHGEF18 antibody; ARHGEF18; Rho guanine nucleotide exchange factor 18;
The ARHGEF18 arhgef18 (Catalog #MBS715731) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Rho guanine nucleotide exchange factor 18 polyclonal Antibody, FITC reacts with Human. Other species are not tested. Please decide the specificity by homology. and may cross-react with other species as described in the data sheet. MyBioSource\'s Rho guanine nucleotide exchange factor 18 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the ARHGEF18 arhgef18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The ARHGEF18 arhgef18 product has the following accession number(s) (GI #195972857) (NCBI Accession #NP_001124427.1) (Uniprot Accession #Q6ZSZ5). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti-human Rho guanine nucleotide exchange factor 18 polyclonal Antibody, FITC with the following immunoassay(s):
Western Blot (WB) (Western blot All lanes: Rho guanine nucleotide exchange factor 18 antibody at at 2 ug/ml Lane 1: EC109whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 129 kDa Observed band size: 30 kDa Additional bands at: 60kDa. We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include.. · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). p114RgoGEF cts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. It may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It also act as a GEF for RAC1, inducing production of reactive oxygen species (ROS), but does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production.