anti-ARNT2 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-ARNT2 antibody; ARNT2;
The ARNT2 arnt2 (Catalog #MBS9128732) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARNT2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s ARNT2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).WB 1:500 - 1:2000
IHC 1:50 - 1:200. Researchers should empirically determine the suitability of the ARNT2 arnt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ARNT2 arnt2 product has the following accession number(s) (GI #68303555) (NCBI Accession #NP_055677.3) (Uniprot Accession #Q9HBZ2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ARNT2 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using ARNT2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney using ARNT2 antibody at dilution of 1:100 (40x lens).)
This gene encodes a member of the basic-helix-loop-helix-Per-Arnt-Sim (bHLH-PAS) superfamily of transcription factors. The encoded protein acts as a partner for several sensor proteins of the bHLH-PAS family, forming heterodimers with the sensor proteins that bind regulatory DNA sequences in genes responsive to developmental and environmental stimuli. Under hypoxic conditions, the encoded protein complexes with hypoxia-inducible factor 1alpha in the nucleus and this complex binds to hypoxia-responsive elements in enhancers and promoters of oxygen-responsive genes. A highly similar protein in mouse forms functional complexes with both aryl hydrocarbon receptors and Single-minded proteins, suggesting additional roles for the encoded protein in the metabolism of xenobiotic compounds and the regulation of neurogenesis, respectively.
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human ARNT2. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ARNT2 are readily searchable from our website. Different antibodies against the same target such as ARNT2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. ARNT2 also interacts with the following gene(s): AHR, CYP1A1, CYP1A2, CYP1B1, EPAS1, HIF1A, NPAS4, POU3F2.