anti-AT3G16565 antibody product blog
Tags: Antibody; Polyclonal Antibody; tRNA Synthetase, Alanyl; AT3G16565; anti-AT3G16565 antibody;
The AT3G16565 n/a (Catalog #MBS628242) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The tRNA Synthetase, Alanyl, NT (AlaRS, Alanyl-tRNA Synthetase, Cytoplasmic, Alanine-tRNA Ligase, AlaRS, Renal Carcinoma Antigen NY-REN-42, AARS) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s tRNA Synthetase, Alanyl can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50. Researchers should empirically determine the suitability of the AT3G16565 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The AT3G16565 n/a product has the following accession number(s) (GI #145361275) (NCBI Accession #NP_683570.3). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the tRNA Synthetase, Alanyl, NT (AlaRS, Alanyl-tRNA Synthetase, Cytoplasmic, Alanine-tRNA Ligase, AlaRS, Renal Carcinoma Antigen NY-REN-42, AARS) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of anti-AARS Pab in K562 cell line lysates (35ug/lane).AARS (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AARS antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases,of the class II enzymes.Class II tRNA synthases evolved early in evolution and are highly conserved.This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein.tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons.They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.
Immunogen: KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa68-98 from the N-terminal region of human AARS. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing AT3G16565 are readily searchable from our website. Different antibodies against the same target such as AT3G16565 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.