anti-Atp5b antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-ATP5B antibody; ATP5B;
The Atp5b atp5b (Catalog #MBS627853) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATP5B, ID (ATP Synthase Subunit beta, Mitochondrial, ATPSB, ATPMB) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s ATP5B can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS).Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry.
Dilution: ELISA: 1:1000
Western Blot: 1:100-1:500
Flow Cytometry: 1:10-1:50
Immunohistochemistry: 1:50-1:100. Researchers should empirically determine the suitability of the Atp5b atp5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Atp5b atp5b product has the following accession number(s) (GI #23272966) (Uniprot Accession #P56480). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ATP5B, ID (ATP Synthase Subunit beta, Mitochondrial, ATPSB, ATPMB) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of ATP5B Antibody (Center) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in WiDr cell line lysate. ATP5B (arrow) was detected using the purified Pab.)
Western Blot (WB) (Western blot analysis of ATP5B (arrow) using rabbit polyclonal ATP5B Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATP5B gene (Lane 2).)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human brain tissue reacted with ATP5B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (ATP5B Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). It is the beta subunit of the catalytic core.