anti-ATP5D antibody product blog
Tags: Antibody; Polyclonal Antibody; ATP5D; anti-ATP5D antibody;
The ATP5D atp5d (Catalog #MBS7044249) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATP5D Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s ATP5D can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the ATP5D atp5d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The ATP5D atp5d product has the following accession number(s) (GI #50345991) (NCBI Accession #NP_001001975.1) (Uniprot Accession #P30049). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ATP5D Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot
All lanes: ATP5D antibody at 1.94 ug/ml
Lane 1: Raji whole cell lysate
Lane 2: NIH/3T3 whole cell lysate
Lane 3: A549 whole cell lysate
Lane 4: MDA-MB-231 whole cell lysate
Lane 5: HepG-2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human brain tissue using MBS7044249 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human lung cancer using MBS7044249 at dilution of 1:100)
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Species: Human
Immunogen: Recombinant human ATP synthase subunit delta, mitochondrial protein (1-168AA). Conjugate: Non-conjugated
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ATP5D are readily searchable from our website. Different antibodies against the same target such as ATP5D may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.