anti-ATP5F1 antibody product blog
Tags: Antibody; Polyclonal Antibody; ATP5F1; anti-ATP5F1 antibody; ATP synthase F (0) complex subunit B1;
The ATP5F1 atp5f1 (Catalog #MBS1497557) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human ATP synthase F(0) complex subunit B1, mitochondrial polyclonal Antibody(ATP5F1) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s ATP synthase F (0) complex subunit B1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC).Recommended dilution: WB:1:500-2000
IHC:1:20-1:200. Researchers should empirically determine the suitability of the ATP5F1 atp5f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ATP5F1 atp5f1 product has the following accession number(s) (GI #21361565) (NCBI Accession #NP_001679.2) (Uniprot Accession #P24539). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti-human ATP synthase F(0) complex subunit B1, mitochondrial polyclonal Antibody(ATP5F1) with the following immunoassay(s):
Western Blot (WB) (All lanes: ATP synthase F(0) complex subunit B1, mitochondrial antibody at 4ug/ml
Lane 1:mouse heart tissue
Lane 2:mouse skeletal muscle tissue
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 29kDa
Observed band size: 29kDa
)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon cancer using MBS1497557 at dilution 1:100)
Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha3beta3 subcomplex and subunit a/ATP6 static relative to the rotary elements.
Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate: Non-conjugated. Immunogen: Recombinant human ATP synthase F(0) complex subunit B1, mitochondrial protein (1-245aa)
Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-162552 / sc-162553. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ATP5F1 are readily searchable from our website. Different antibodies against the same target such as ATP5F1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.