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anti-AurB antibody product blog

Posted on 2016-05-04 22:53:47 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; AurB; anti-AurB antibody;
The AurB aurkb (Catalog #MBS9403540) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AurB (Phospho-Thr232) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s AurB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).
Western blotting: 1:500~1:1000. Researchers should empirically determine the suitability of the AurB aurkb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The AurB aurkb product has the following accession number(s) (GI #378786658) (NCBI Accession #NP_001243763.1) (Uniprot Accession #Q96GD4). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the AurB (Phospho-Thr232) Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts from COS7 cells treated with Nocodazole using Phospho-AurB (Thr232) antibody.The lane on the right is treated with the antigen-specific peptide.)
Western Blot (WB) AurB.

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Caldas GV, DeLuca KF, DeLuca JG (2013)J Cell Biol 203, 957-69. Zhang L, et al. (2012)J Biol Chem 287, 34069-77. Ratushny V, et al. (2012)Oncogene 31, 1217-27.

Immunogen Type: Peptide-KLH
Immunogen Description: Peptide sequence around phosphorylation site of threonine 232 (R-K-T(p)-M-C) derived from Human AurB. Modification: Phospho-Thr232
Target Name: AurB. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing AurB are readily searchable from our website. Different antibodies against the same target such as AurB may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. AurB also interacts with the following gene(s): BIRC5, CDCA8, CENPA, INCENP, KLHL13, KLHL9, MAPRE1, NDC80, RACGAP1.
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