anti-AXIN1 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-AXIN1 antibody; AXIN1;
The AXIN1 axin1 (Catalog #MBS6001573) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AXIN1, CT (AXIN1, AXIN, Axin-1, Axis inhibition protein 1) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s AXIN1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50. Researchers should empirically determine the suitability of the AXIN1 axin1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The AXIN1 axin1 product has the following accession number(s) (GI #31083144) (NCBI Accession #NP_851393.1) (Uniprot Accession #O15169). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the AXIN1, CT (AXIN1, AXIN, Axin-1, Axis inhibition protein 1) with the following immunoassay(s):
Western Blot (WB) (AXIN1 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the AXIN1 antibody detected the AXIN1 protein (arrow).)
Immunohistochemistry (IHC) (AXIN1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AXIN1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Two transcript variants encoding distinct isoforms have been identified for this gene.
Immunogen: AXIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 717-746 amino acids from the C-terminal region of human AXIN1. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing AXIN1 are readily searchable from our website. Different antibodies against the same target such as AXIN1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.