anti-BAX antibody product blog
Tags: Antibody; anti-Bax antibody; Bax; Polyclonal Antibody;
The BAX bax (Catalog #MBS125008) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Bax Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Bax can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).WB: 1:500 - 1:2000
IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the BAX bax for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The BAX bax product has the following accession number(s) (GI #728945) (NCBI Accession #Q07812.1) (Uniprot Accession #Q07812). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Bax Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of Raji cells, using BAX antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse lung using Bax antibody at dilution of 1:100 (40x lens).)
Bax is a key component for cellular induced apoptosis through mitochondrial stress (1). Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane (2). Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane\'s permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9 and initiation of the caspase activation pathway for apoptosis (3,4).
Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human Bax
Calculated Molecular Weight: 21kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing BAX are readily searchable from our website. Different antibodies against the same target such as BAX may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Brain Diseases, Breast Neoplasms, Heart Diseases, Inflammation, Intestinal Neoplasms, Ischemia, Liver Diseases, Necrosis, Neoplasms, Experimental, Nervous System Diseases are some of the diseases may be linked to Bax Polyclonal Antibody. The following patways have been known to be associated with this gene. Blood, Bone, Brain, Eye, Liver, Lung, Nerve, Prostate, Skin, Stomach tissues are correlated with this protein.