anti-BCAR1 antibody product blog
Tags: Antibody; Polyclonal Antibody; BCAR1; anti-BCAR1 antibody;
The BCAR1 bcar1 (Catalog #MBS423291) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-BCAR1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. MyBioSource\'s BCAR1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC).Peptide ELISA: Antibody detection limit dilution 1: 128000.
Immunohistochemistry: In paraffin embedded Human Uterus shows strong staining at the glandular side of the endometrial epithelium. Recommended concentration, 5-10ug/ml.
Western Blot: Approx 100kDa band observed in lysates of Human breast cancer and of cell line MCF7 (calculated MW of 97.9kDa according to NP_001164185.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the BCAR1 bcar1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The BCAR1 bcar1 product has the following accession number(s) (GI #282398112) (NCBI Accession #NP_001164185.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
KQTSTFSKQT PHH.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-BCAR1 Antibody with the following immunoassay(s):
Western Blot (WB) ((1ug/ml) staining of MCF7 (A) and Human Breast cancer (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC) ((5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunogen: Peptide with sequence C-KQTSTFSKQTPHH, from the internal region of the protein sequence according to NP_001164185.1; NP_001164186.1; NP_001164187.1; NP_001164188.1; NP_001164189.1; NP_055382.2; NP_001164190.1; NP_001164191.1; NP_001164192.1.
Epitope: Internal region. Note: This antibody is expected to recognize reported isoforms1, 2, 3, 5, 6 and 7. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing BCAR1 are readily searchable from our website. Different antibodies against the same target such as BCAR1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Bone, Brain, Heart, Kidney, Liver, Lung, Muscle, Pancreas, Prostate, Vascular tissues are correlated with this protein. BCAR1 also interacts with the following gene(s): CRK, CRKL, DOCK1, FYN, PTK2, PTK2B, PTPN1, PXN, SRC.