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anti-BECN1 antibody product blog

Posted on 2015-11-22 06:40:24 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; Beclin-1; anti-BECN1 antibody; BECN1;
The BECN1 becn1 (Catalog #MBS151201) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Beclin-1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Beclin-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF).
Beclin-1 antibody can be used for the detection of Beclin-1 by Western blot at 0.5 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 1 mug/mL. For immunofluorescence start at 2 mug/mL. Researchers should empirically determine the suitability of the BECN1 becn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The BECN1 becn1 product has the following accession number(s) (GI #16307457) (NCBI Accession #AAH10276) (Uniprot Accession #Q14457). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Beclin-1 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of Beclin-1 in A431 cell lysate with Beclin-1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.)
Western Blot (WB) BECN1.

Immunocytochemistry (ICC) (Immunocytochemistry staining of A431 cells using Beclin-1 antibody at 1 μg/mL.)
Immunocytochemistry (ICC) BECN1.

Immunofluorescence (IF) (Immunofluorescence of Beclin-1 in A431 cells with Beclin-1 antibody at 2 μg/mL.)
Immunofluorescence (IF) BECN1.

Beclin-1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death.

Conjugate: Unconjugated
Immunogen: Beclin-1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Beclin-1.
Buffer: Beclin-1 Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing BECN1 are readily searchable from our website. Different antibodies against the same target such as BECN1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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