anti-BHLHE22 antibody product blog
Tags: Antibody; Polyclonal Antibody; BHLHE22; BHLHB5; anti-BHLHE22 antibody;
The BHLHE22 bhlhe22 (Catalog #MBS643072) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BHLHB5, ID (BHLHE22, BHLHB5, TNRC20, Class E basic helix-loop-helix protein 22, Class B basic helix-loop-helix protein 5, Trinucleotide repeat-containing gene 20 protein) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s BHLHB5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS).Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the BHLHE22 bhlhe22 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The BHLHE22 bhlhe22 product has the following accession number(s) (GI #119607282) (NCBI Accession #EAW86876.1) (Uniprot Accession #Q8NFJ8). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the BHLHB5, ID (BHLHE22, BHLHB5, TNRC20, Class E basic helix-loop-helix protein 22, Class B basic helix-loop-helix protein 5, Trinucleotide repeat-containing gene 20 protein) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of BHLHB5 Antibody (Center) in HL-60 cell line lysates (35ug/lane). BHLHB5 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (BHLHB5 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BHLHB5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (BHLHB5 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
BHLHB5 inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. BHLHB5 despite the presence of an intact basic domain, does not bind to DNA.
Immunogen: BHLHB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243~272 amino acids from the Central region of human BHLHB5. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing BHLHE22 are readily searchable from our website. Different antibodies against the same target such as BHLHE22 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.