anti-BMF antibody product blog
Tags: Antibody; Polyclonal Antibody; BMF; anti-BMF antibody;
The BMF bmf (Catalog #MBS151560) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Bmf Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Bmf can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC).Bmf antibody can be used for detection of Bmf by Western blot at 2 mug/mL. A band at approximately 25 kDa can be detected. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. Researchers should empirically determine the suitability of the BMF bmf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The BMF bmf product has the following accession number(s) (GI #15723378) (NCBI Accession #NP_277038) (Uniprot Accession #Q96LC9). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Bmf Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of Bmf in 293 cell lysate with Bmf antibody at (A) 1 and (B) 2 μg/ml.)
Immunocytochemistry (IHC) (Immunocytochemistry of Bmf in HeLa cells with Bmf antibody at 10 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of Bmf in HeLa cells with Bmf antibody at 5 μg/mL.
Red: Bmf Antibody (3031)
Blue: DAPI staining)
Bmf Antibody: Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell\'s actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program. Bmf is constitutively expressed in many tissues.
Conjugate: Unconjugated
Immunogen: Bmf antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human Bmf.
Buffer: Bmf Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing BMF are readily searchable from our website. Different antibodies against the same target such as BMF may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.