anti-BRCA1 antibody product blog
Tags: Antibody; Polyclonal Antibody; BRCA1; anti-BRCA1 antibody;
The BRCA1 brca1 (Catalog #MBS176954) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-BRCA1 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s BRCA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Researchers should empirically determine the suitability of the BRCA1 brca1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The BRCA1 brca1 product has the following accession number(s) (GI #728984) (NCBI Accession #P38398.2) (Uniprot Accession #P38398). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-BRCA1 antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-BRCA1 Picoband antibody, MBS176954-1.JPG
IHC(P): Mouse Testis Tissue )
Immunohistochemistry (IHC) (Anti-BRCA1 Picoband antibody, MBS176954-2.JPG
IHC(P): Rat Testis Tissue )
Immunohistochemistry (IHC) (Anti-BRCA1 Picoband antibody, MBS176954-3.JPG
IHC(P): Human Mammary Cancer Tissue )
Testing Data (Anti-BRCA1 Picoband antibody, MBS176954-4.jpg
All lanes: Anti-BRCA1(MBS176954) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Predicted bind size: 207KD
Observed bind size: 207KD )
Description: Rabbit IgG polyclonal antibody for Breast cancer type 1 susceptibility protein(BRCA1) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: BRCA1, also known as BRCC1, is a gene which mapping to 17q21.3. This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). BRCA1 product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. In addition to it, BRCA1 may normally serve as a negative regulator of mammary epithelial cell growth and that this function is compromised in breast cancer either by direct mutation or by alterations in gene expression.
Immunogen: E Coli-derived human BRCA1 recombinant protein (Position: E1661-Y1863). Human BRCA1 shares 65% and 66% amino acid (aa) sequence identity with mouse and rat BRCA1, respectively. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing BRCA1 are readily searchable from our website. Different antibodies against the same target such as BRCA1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.