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anti-C13orf34 antibody product blog

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Posted on 2016-04-02 05:42:30 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; Bora; anti-C13orf34 antibody; C13orf34;
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The C13orf34 bora (Catalog #MBS151472) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Bora Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Bora can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).
Bora antibody can be used for detection of Bora by Western blot at 1-2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the C13orf34 bora for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The C13orf34 bora product has the following accession number(s) (GI #74737659) (NCBI Accession #Q6PGQ7.2) (Uniprot Accession #Q6PGQ7). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Bora Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of Bora in Jurkat cell lysate with Bora antibody at (A) 1 and (B) 2 μg/mL.)
Western Blot (WB) C13orf34.

Immunohistochemistry (IHC) (Immunohistochemistry of Bora in mouse brain tissue with Bora antibody at 2.5 μg/mL.)
Immunohistochemistry (IHC) C13orf34.

Immunofluorescence (IF) (Immunofluorescence of Bora in Mouse Brain cells with Bora antibody at 20 μg/mL.)
Immunofluorescence (IF) C13orf34.

Bora Antibody: Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.

Conjugate: Unconjugated
Immunogen: Bora antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human Bora.
Buffer: Bora Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing C13orf34 are readily searchable from our website. Different antibodies against the same target such as C13orf34 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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