anti-CALCRL antibody product blog
Tags: Antibody; Polyclonal Antibody; CALCRL; Calcitonin Receptor Like Receptor; anti-CALCRL antibody;
The CALCRL calcrl (Catalog #MBS611175) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Calcitonin Receptor Like Receptor (CRLR) reacts with Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Calcitonin Receptor Like Receptor can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000-1:5000 (ECL).
ELISA: 1:10,000-1:50,000. Control peptide can be used to coat plates at 1ug/ml. Researchers should empirically determine the suitability of the CALCRL calcrl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CALCRL calcrl product has the following accession number(s) (GI #5031621) (NCBI Accession #NP_005786.1) (Uniprot Accession #Q16602). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
The calcitonin family peptides probably act through G-protein coupled membrane receptors. The gene for calcitonin receptors has been cloned. It is homologous to GPCRs in family "B" which typically recognizes regulatory peptides (secretin, glucagons, VIP). Recently, a homolog of calcitonin receptor, CRLR (calcitonin-receptor-like receptor human 461aa; rat/mouse 463aa) was identified. CRLR has 55% homology with calcitonin receptor. It is now shown that CRLR can function as either a CGRP receptor or an ADM receptor, depending upon which members of a new family of proteins called receptor activity modifying proteins (RAMP1-3) are expressed. RAMPs1-3 contains an N-terminal signal peptide, an extracellular N-terminus, a single transmembrane domain near the C-terminus, and cytoplasmic C-terminus. RAMP1-3 displays 31% identity. RAMPS may be involved in the transport of CRLR to the plasma membrane. RAMP1 (human, mouse, rat 148 aa) presents the CRLR receptor as a glycoprotein that function as CGRP receptor. RAMP1 is expressed in many tissues, including the uterus, bladder, brain, pancreas, and GI tract. CRLR and RAMP1 are not co-expressed in all tissues suggesting that their co-expression may define which cells express functional CGRP receptors. RAMP2 (human 175aa; rat 182aa, and mouse 189 aa)-transported receptors are core-glycosylated and function as ADM receptor. It is expressed in the lung, breast, immune system and fetal tissues. RAMP3 is most abundant in the kidney and lung.
Immunogen: Synthetic peptide consisting of 20aa mapping near the cytoplasmic, C-terminus of rat CRLR (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CALCRL are readily searchable from our website. Different antibodies against the same target such as CALCRL may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.