anti-CARM1 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-Carm1 antibody; Carm1; PRMT4;
The CARM1 carm1 (Catalog #MBS6005153) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRMT4, CT (Protein Arginine N-Methyltransferase 4, CARM1) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PRMT4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50. Researchers should empirically determine the suitability of the CARM1 carm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CARM1 carm1 product has the following accession number(s) (GI #530414438) (NCBI Accession #XP_005259765.1) (Uniprot Accession #Q86X55). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PRMT4, CT (Protein Arginine N-Methyltransferase 4, CARM1) with the following immunoassay(s):
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with PRMT4 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.
Immunogen: PRMT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PRMT4. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CARM1 are readily searchable from our website. Different antibodies against the same target such as CARM1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.