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anti-CAS antibody product blog

Posted on 2016-07-16 16:07:51 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; CAS; anti-CAS antibody;
The CAS cse1l (Catalog #MBS301006) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti-Human CAS Polyclonal Antibody reacts with Human
Predicted Reactivity: Bovine, Frog, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s CAS can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin.
Immunohistochemistry Procedure
Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
Immunohistochemistry Dilution: 1:100
IHC Positive Control: Liver Carcinoma. Researchers should empirically determine the suitability of the CAS cse1l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CAS cse1l product has the following accession number(s) (GI #371502112) (NCBI Accession #NP_001243064.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit Anti-Human CAS Polyclonal Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Human liver carcinoma stained with anti-CAS antibody)
Immunohistochemistry (IHC) CAS.

CAS (Cellular Apoptosis Susceptibility) contains 971 amino acids with 59% overall sequence homology as compared to yeast chromosome segregation homolog, CSE1. CAS is highly expressed in testis and fetal liver. CAS expression in human fibroblasts increases as cells are induced to proliferate and decreases in growth arrested cells.

Immunogen: Synthetic peptide from N-terminus of human CAS.
Control Tissue: Liver Carcinoma
Cellular Localization: Cytoplasm
Entrez Gene Code: 1434
Pathway: Tyrosine Kinase/Adaptors. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CAS are readily searchable from our website. Different antibodies against the same target such as CAS may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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