anti-CASP1 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-CASP1 antibody; CASP1; Caspase-1(P20);
The CASP1 casp1 (Catalog #MBS176241) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Anti-CASP1 (p20) Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Caspase-1(P20) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).Western Blot:
Concentration: 0.1-0.5ug/ml
Tested Species: Ms, Rat
Predicted Species: -
Antigen Retrieval: -
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the CASP1 casp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CASP1 casp1 product has the following accession number(s) (GI #266322) (NCBI Accession #P29452.1) (Uniprot Accession #P29452). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Polyclonal Anti-CASP1 (p20) Antibody with the following immunoassay(s):
Western Blot (WB) (Anti-Caspase-1(P20) antibody, MBS176241, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: Mouse Spleen Tissue Lysate
Lane 5: Mouse Testis Tissue Lysate
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Caspase 1 is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta, IL18, and IL33 precursor proteins. It belongs to a family of cysteine proteases known as caspases that always cleave proteins following an aspartic acid residue. The Caspase1 gene consists of 10 exons spanning at least 10.6 kb. The Caspase 1 gene is mapped to 11q23, a site frequently involved in rearrangement in human cancers, including a number of leukemias and lymphomas, by Southern DNA blot analysis of rodent-human hybrids and by in situ hybridization to normal human metaphase chromosomes. Caspase 1 has been shown to induce cell necrosis or pyroptosis and may function in various developmental stages.
Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse CASP1 (137-157aa LEKAQKLWKENPSEIYPIMNT), different from the related rat sequence by three amino acids. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems: MBS provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit MBS176460 in WB. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CASP1 are readily searchable from our website. Different antibodies against the same target such as CASP1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.