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anti-CASP3 antibody product blog

Posted on 2017-03-04 02:09:40 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; CASP3; anti-CASP3 antibody;
The CASP3 casp3 (Catalog #MBS2519424) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CASP3 (active) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s CASP3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC).
WB: 1:200-1:1000
IHC: 1:25-1:100. Researchers should empirically determine the suitability of the CASP3 casp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CASP3 casp3 product has the following accession number(s) (GI #77416852) (NCBI Accession #P42574.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CASP3 (active) Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Jurkat and MCF7 cell using CASP3 (active) Polyclonal Antibody at dilution of 1:120)
Western Blot (WB) CASP3.

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded Human thyroid cancer using CASP3 (active) Polyclonal Antibody at dilution of 1:15)
Immunohistochemistry (IHC) CASP3.

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer\'s disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Immunogen: Synthetic peptide of human CASP3 (active)
Calculated Molecular Weight: 17kDa. Buffer: PBS with 0.05% sodium azide, 50% glycerol, pH7.3. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CASP3 are readily searchable from our website. Different antibodies against the same target such as CASP3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Blood, Bone, Brain, Heart, Kidney, Liver, Lung, Muscle, Prostate, Vascular tissues are correlated with this protein.
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