anti-CASP8 antibody product blog
Tags: Antibody; CASP8; Polyclonal Antibody; anti-CASP8 antibody; Caspase-8;
The CASP8 casp8 (Catalog #MBS151342) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Caspase-8 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Caspase-8 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF).Caspase-8 antibody can be used for the detection of caspase-8 by Western blot at 0.5 to 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 2 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the CASP8 casp8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CASP8 casp8 product has the following accession number(s) (GI #15718704) (NCBI Accession #NP_001219) (Uniprot Accession #Q14790). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Caspase-8 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of caspase-8 in Jurkat cell lysate with caspase-8 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Immunohistochemistry (IHC) (Immunohistochemistry of Caspase-8 in human spleen tissue with Caspase-8 antibody at 5 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of Caspase-8 in Jurkat cells with Caspase-8 antibody at 20 μg/mL.)
Immunocytochemistry (ICC) (Immunocytochemistry of caspase-8 in Jurkat cells with caspase-8 antibody at 2 μg/mL.)
Western Blot (WB) (Western blot analysis of Caspase-8 in (A) human spleen, (B) mouse spleen, and (C) rat spleen tissue lysate with Caspase-8 antibody at 0.5 μg/ml.)
Immunofluorescence (IF) (Immunofluorescence of Caspase-8 in human spleen tissue with Caspase-8 antibody at 20 μg/ml.
Red: Caspase-8 Antibody (3473)
Blue: DAPI staining)
Caspase-8 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-8 is an initiator caspase that was identified as a member of the Fas/APO-1 death-inducing signaling complex. The adaptor molecule FADD couples procaspase-8 to the Fas receptor death domain; subsequent oligomerization promotes procaspase-8 autoactivation. FLIP, a catalytically inactive caspase-8-like molecule inhibits these interactions and thus can inhibit apoptosis.