anti-CASP8 antibody product blog
Tags: Antibody; CASP8; Polyclonal Antibody; anti-CASP8 antibody; Caspase-8;
The CASP8 casp8 (Catalog #MBS224284) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI CASPASE-8 (C-TERMINAL) reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s CASPASE-8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).Western Blot: This item detects a cleaved subunit band of approximately 22.5kDa in Jurkat cell lysates (predicted precursor MWt 57.7kDa).
Western Blotting: Maximum Dilution: 0.5ug/ml. Researchers should empirically determine the suitability of the CASP8 casp8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CASP8 casp8 product has the following accession number(s) (GI #122056474) (NCBI Accession #NP_001073593.1) (Uniprot Accession #Q14790). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RABBIT ANTI CASPASE-8 (C-TERMINAL) with the following immunoassay(s):
Testing Data #1 (Western blot analysis of lysate from Jurkat human T-cell leukemia cells probed with Rabbit anti Human caspase-8 (MBS224284) at 0.5(A), 1(B) and 2(C) ug/ml)
Testing Data #2 (Immunocytochemical staining of Jurkat cells with Rabbit anti Human caspase-8 (MBS224284))
MBS224284 recognises an epitope within the c-terminal region (CT) of caspase-8, otherwise known as Fas-linked ICE-like protease (FLICE), which shares homology with both Fas-associated via death domain (FADD) and the ICE/Ced-3 family of cysteine proteases. Caspase-8 is classified as an initiator caspase and acts as the most upstream trigger of Fas-Receptor (CD95) and tumour necrosis factor receptor 1 (TNFR1) mediated apoptosis. Upon activation, the cell death receptor Fas, links with the adaptor molecule FADD via corresponding death domains (DD). FADD in turn then binds via death effector domains (DEDs) to procaspase-8, which then undergoes oligomerization and autocatalytic activation. Active Caspase-8 is capable of cleaving all other known caspases, and also cleaves the Bcl-2 family member BH3-interacting domain death agonist (BID), along with the Bcl-2 associated protein p28Bap31, see Wolf, B. B. & Green, D. R. for details.
Perservative Stabilisers: 0.02% Sodium Azide. Immunogen: A 16 amino acid peptide located near the carboxy-terminus of human caspase-8 isoform A.
Buffer Solution: Phosphate buffered saline
Antiserum Preparation: Antisera to human caspase-8 (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CASP8 are readily searchable from our website. Different antibodies against the same target such as CASP8 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.