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anti-casp9 antibody product blog

Posted on 2016-06-05 05:54:19 by mybiosource_staff
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Tags: Antibody; CASP9; Polyclonal Antibody; anti-CASP9 antibody; Caspase 9, Cleavage Site;
The casp9 n/a (Catalog #MBS613144) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Caspase 9, Cleavage Site (Asp330) (ICE-LAP6, Mch6) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Caspase 9, Cleavage Site can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP).
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:50
Immunocytochemistry (ABC) 1:100
Flow cytometry 1:10. Researchers should empirically determine the suitability of the casp9 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The casp9 n/a product has the following accession number(s) (GI #148233858) (NCBI Accession #NP_001079035.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47kD)/Apaf 1. This complex processes procaspase-9 into a large active subunit (35kD or 17kD) and a small subunit (10kD) by self-cleavage at Asp315 (3-5). Cleaved caspase-9 further processes other caspase members, including caspase- 3 and caspase-7, to initiate a caspase cascade, leading to programmed cell death (6-9). In addition to self-cleavage, procaspase-9 also can be cleaved in vivo by caspase-3 at Asp330. This process serves as positive feedback to amplify the apoptotic signal in the caspase activation pathway (3-5).

casp9 n/a

Immunogen: Synthetic peptide corresponding to amino terminus residues adjacent to Asp330 of human Caspase 9. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing casp9 are readily searchable from our website. Different antibodies against the same target such as casp9 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Bone, Brain, Connective Tissue, Ear, Embryonic Tissue, Heart, Kidney, Muscle, Umbilical Cord, Vascular tissues are correlated with this protein.
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