anti-CASR antibody product blog
Tags: Antibody; anti-CASR antibody; CASR; Polyclonal Antibody;
The CASR casr (Catalog #MBS153366) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CASR Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s CASR can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).CASR antibody can be used for detection of CASR by Western blot at 1 - 2 mug/ml. Antibody can also be used for Immunohistochemistry at 5 mug/mL. For Immunoflorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the CASR casr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CASR casr product has the following accession number(s) (GI #296010811) (NCBI Accession #NP_001171536) (Uniprot Accession #P41180). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CASR Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of CASR in EL4 cell lysate with CASR antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Immunohistochemistry (IHC) (Immunohistochemistry of CASR in human kidney tissue with CASR antibody at 5 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of CASR in human kidney tissue with CASR antibody at 20 μg/mL.)
The calcium-sensing receptor protein (CASR) is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH)-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule (1). It senses small changes in circulating calcium concentration and couples this information to intracellular signaling pathways that modify PTH secretion or renal cation handling, thus this protein plays an essential role in maintaining mineral ion homeostasis (1). Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism (1,2). Recent evidence suggests that activated CASR contributes to the cytokine secretion through the partial MAPK and NF-kappaB pathways in T cells (3).
Conjugate: Unconjugated
Immunogen: CASR antibody was raised against a 15 amino acid peptide near the amino terminus of human CASR.
Buffer: CASR Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CASR are readily searchable from our website. Different antibodies against the same target such as CASR may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.