anti-CBLC antibody product blog
Tags: Antibody; Polyclonal Antibody; CBLC; anti-CBLC antibody; E3 ubiquitin-protein ligase CBL-C;
The CBLC cblc (Catalog #MBS1491737) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human E3 ubiquitin-protein ligase CBL-C polyclonal Antibody(CBLC) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s E3 ubiquitin-protein ligase CBL-C can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC).Recommended dilution: WB:1:500-2000
IHC:1:20-1:200. Researchers should empirically determine the suitability of the CBLC cblc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CBLC cblc product has the following accession number(s) (GI #195927030) (NCBI Accession #NP_001124324.1) (Uniprot Accession #Q9ULV8). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti-human E3 ubiquitin-protein ligase CBL-C polyclonal Antibody(CBLC) with the following immunoassay(s):
Western Blot (WB) (All lanes: E3 ubiquitin-protein ligase CBL-C antibody at 14ug/ml
Lane 1:A549 whole cell lysate
Lane 2:Hela whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 53,48 kDa
Observed band size: 52 kDa
)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human breast cancer using MBS1491737 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human pancreas tissue using MBS1491737 at dilution 1:100)
Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Functionally coupled with the E2 ubiquitin-protein ligases UB2D1, UB2D2 and UB2D3. Regulator of EGFR mediated signal transduction; upon EGF activation, ubiquitinates EGFR. Isoform 1, but not isoform 2, inhibits EGF stimulated MAPK1 activation. Promotes ubiquitination of SRC phosphorylated at \'Tyr-419\'. In collaboration with CD2AP may act as regulatory checkpoint for Ret signaling by modulating the rate of RET degradation after ligand activation; CD2AP converts it from an inhibitor to a promoter of RET degradation; the function limits the potency of GDNF on neuronal survival.
Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate: Non-conjugated. Immunogen: Recombinant human E3 ubiquitin-protein ligase CBL-C protein (245-474aa)
Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-8372 / sc-8371. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CBLC are readily searchable from our website. Different antibodies against the same target such as CBLC may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.