anti-CCL11 antibody product blog
Tags: Antibody; Polyclonal Antibody; CCL11; anti-CCL11 antibody;
The CCL11 ccl11 (Catalog #MBS9128939) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CCL11 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s CCL11 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF).WB 1:500 - 1:2000
IF 1:50 - 1:100. Researchers should empirically determine the suitability of the CCL11 ccl11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CCL11 ccl11 product has the following accession number(s) (GI #4506827) (NCBI Accession #NP_002977.1) (Uniprot Accession #P51671). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CCL11 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using CCL11 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat lung using CCL11 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat brain using CCL11 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human stomach using CCL11 antibody at dilution of 1:100 (40x lens).)
Immunofluorescence (IF) (Immunofluorescence analysis of A549 cells using CCL11 antibody. Blue: DAPI for nuclear staining.)
This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, displays chemotactic activity for eosinophils, but not mononuclear cells or neutrophils. This eosinophil-specific chemokine is thought to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections.
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human CCL11. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CCL11 are readily searchable from our website. Different antibodies against the same target such as CCL11 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.