anti-CCL2 antibody product blog
Tags: Antibody; Polyclonal Antibody; CCL2; anti-CCL2 antibody;
The CCL2 ccl2 (Catalog #MBS9207814) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CCL2 Antibody (C-term) reacts with Human (Predicted Reactivity: Monkey) and may cross-react with other species as described in the data sheet. MyBioSource\'s CCL2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS).WB~~1:1000
IF~~1:10~50
IHC-P~~1:10~50
FC~~1:10~50. Researchers should empirically determine the suitability of the CCL2 ccl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CCL2 ccl2 product has the following accession number(s) (GI #4506841) (NCBI Accession #NP_002973.1) (Uniprot Accession #P13500). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CCL2 Antibody (C-term) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of CCL2 Antibody (C-term) in Hela cell line lysates (35ug/lane). CCL2 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human brain tissue with CCL2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunofluorescence (IF) (Immunofluorescence analysis of CCL2 Antibody (C-term) with hela cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of CCL2 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of hela cells using CCL2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. CCL2 is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
Antigen Type: Synthetic Peptide. Function: Acts as a ligand for C-C chemokine receptor CCR2 (PubMed:9837883, PubMed:10587439, PubMed:10529171). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed:9837883, PubMed:10587439). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed:8627182, PubMed:9792674, PubMed:8195247). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed:8107690).
Cellular Location: Secreted
Tissue Location: Expressed in the seminal plasma, endometrial fluid and follicular fluid (at protein level) (PubMed:23765988). Expressed in monocytes (PubMed:2513477).